Activation of the Endoperoxide Ascaridole Modulates Its Sensitizing Capacity.
Identifieur interne : 000292 ( PubMed/Checkpoint ); précédent : 000291; suivant : 000293Activation of the Endoperoxide Ascaridole Modulates Its Sensitizing Capacity.
Auteurs : Nora L. Krutz ; Jennifer Hennen ; Corinna Korb ; Mario T. Schellenberger ; G Frank Gerberick [États-Unis] ; Brunhilde Blömeke [Allemagne]Source :
- Toxicological sciences : an official journal of the Society of Toxicology [ 1096-0929 ] ; 2015.
English descriptors
- KwdEn :
- Antigens, CD40 (metabolism), Antigens, CD80 (metabolism), Antigens, CD86 (metabolism), Cell Line, Cytokines (metabolism), Dendritic Cells, Dermatitis, Allergic Contact (etiology), Flow Cytometry, Humans, Intercellular Adhesion Molecule-1 (metabolism), Monoterpenes (adverse effects), Peroxides (adverse effects), Real-Time Polymerase Chain Reaction, Skin (drug effects).
- MESH :
- chemical , adverse effects : Monoterpenes, Peroxides.
- chemical , metabolism : Antigens, CD40, Antigens, CD80, Antigens, CD86, Cytokines, Intercellular Adhesion Molecule-1.
- drug effects : Skin.
- etiology : Dermatitis, Allergic Contact.
- Cell Line, Dendritic Cells, Flow Cytometry, Humans, Real-Time Polymerase Chain Reaction.
Abstract
The monoterpene ascaridole, a fairly stable endoperoxide found in essential oils such as tea tree oil can provoke allergic contact dermatitis which has been evidenced under patch test conditions. However, concomitantly we observed irritative skin reactions that demand further data underlining the sensitization potential of ascaridole. Here, we studied the effects of ascaridole on dendritic cell (DC) activation and protein reactivity, 2 key steps of chemical-induced skin sensitization. Treatment of human monocyte-derived DC with ascaridole found support for full DC maturation, a capability of sensitizers but not irritants. It induced significant upregulation of the expression of the costimulatory molecules CD86, CD80, CD40, and the adhesion molecule CD54 in a time-dependent manner. Maturation was accompanied by release of proinflammatory cytokines interleukin (IL)-1ß, tumor necrosis factor-α, IL-6, and IL-8. Similar to other chemical skin sensitizers including hydroperoxides, we observed a certain reactivity of ascaridole toward cysteine- but not lysine-containing peptides. During recent years, evidence accumulated for a radical mechanism as trigger for protein reactivity of peroxides. Treatment of the fairly stable endoperoxide ascaridole with iron as radical inducer ("activated ascaridole") resulted in cysteine peptide reactivity exceeding by far that of ascaridole itself. Furthermore, activated ascaridole showed increased potential for induction of the Nrf2 target gene heme oxygenase 1 and upregulation of CD86 and CD54 on THP-1 cells, an established DC surrogate. These results indicate that radical formation could be involved in the steps leading to skin sensitization induced by the endoperoxide ascaridole.
DOI: 10.1093/toxsci/kfv148
PubMed: 26185204
Affiliations:
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Krutz</name><affiliation><nlm:affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</nlm:affiliation><wicri:noCountry code="subField">Germany and</wicri:noCountry></affiliation></author><author><name sortKey="Hennen, Jennifer" sort="Hennen, Jennifer" uniqKey="Hennen J" first="Jennifer" last="Hennen">Jennifer Hennen</name><affiliation><nlm:affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</nlm:affiliation><wicri:noCountry code="subField">Germany and</wicri:noCountry></affiliation></author><author><name sortKey="Korb, Corinna" sort="Korb, Corinna" uniqKey="Korb C" first="Corinna" last="Korb">Corinna Korb</name><affiliation><nlm:affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</nlm:affiliation><wicri:noCountry code="subField">Germany and</wicri:noCountry></affiliation></author><author><name sortKey="Schellenberger, Mario T" sort="Schellenberger, Mario T" uniqKey="Schellenberger M" first="Mario T" last="Schellenberger">Mario T. Schellenberger</name><affiliation><nlm:affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</nlm:affiliation><wicri:noCountry code="subField">Germany and</wicri:noCountry></affiliation></author><author><name sortKey="Gerberick, G Frank" sort="Gerberick, G Frank" uniqKey="Gerberick G" first="G Frank" last="Gerberick">G Frank Gerberick</name><affiliation wicri:level="2"><nlm:affiliation>Central Product Safety Department, The Procter & Gamble Company, Mason Business Center, 8700 Mason Montgomery Road, Cincinnati, OH.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Central Product Safety Department, The Procter & Gamble Company, Mason Business Center, 8700 Mason Montgomery Road, Cincinnati</wicri:cityArea></affiliation></author><author><name sortKey="Blomeke, Brunhilde" sort="Blomeke, Brunhilde" uniqKey="Blomeke B" first="Brunhilde" last="Blömeke">Brunhilde Blömeke</name><affiliation wicri:level="1"><nlm:affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and bloemeke@uni-trier.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>*Department of Environmental Toxicology, University Trier, 54296 Trier</wicri:regionArea><wicri:noRegion>54296 Trier</wicri:noRegion><wicri:noRegion>54296 Trier</wicri:noRegion><wicri:noRegion>54296 Trier</wicri:noRegion></affiliation></author></analytic><series><title level="j">Toxicological sciences : an official journal of the Society of Toxicology</title><idno type="eISSN">1096-0929</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, CD40 (metabolism)</term><term>Antigens, CD80 (metabolism)</term><term>Antigens, CD86 (metabolism)</term><term>Cell Line</term><term>Cytokines (metabolism)</term><term>Dendritic Cells</term><term>Dermatitis, Allergic Contact (etiology)</term><term>Flow Cytometry</term><term>Humans</term><term>Intercellular Adhesion Molecule-1 (metabolism)</term><term>Monoterpenes (adverse effects)</term><term>Peroxides (adverse effects)</term><term>Real-Time Polymerase Chain Reaction</term><term>Skin (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Monoterpenes</term><term>Peroxides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, CD40</term><term>Antigens, CD80</term><term>Antigens, CD86</term><term>Cytokines</term><term>Intercellular Adhesion Molecule-1</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Skin</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Dermatitis, Allergic Contact</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Dendritic Cells</term><term>Flow Cytometry</term><term>Humans</term><term>Real-Time Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The monoterpene ascaridole, a fairly stable endoperoxide found in essential oils such as tea tree oil can provoke allergic contact dermatitis which has been evidenced under patch test conditions. However, concomitantly we observed irritative skin reactions that demand further data underlining the sensitization potential of ascaridole. Here, we studied the effects of ascaridole on dendritic cell (DC) activation and protein reactivity, 2 key steps of chemical-induced skin sensitization. Treatment of human monocyte-derived DC with ascaridole found support for full DC maturation, a capability of sensitizers but not irritants. It induced significant upregulation of the expression of the costimulatory molecules CD86, CD80, CD40, and the adhesion molecule CD54 in a time-dependent manner. Maturation was accompanied by release of proinflammatory cytokines interleukin (IL)-1ß, tumor necrosis factor-α, IL-6, and IL-8. Similar to other chemical skin sensitizers including hydroperoxides, we observed a certain reactivity of ascaridole toward cysteine- but not lysine-containing peptides. During recent years, evidence accumulated for a radical mechanism as trigger for protein reactivity of peroxides. Treatment of the fairly stable endoperoxide ascaridole with iron as radical inducer ("activated ascaridole") resulted in cysteine peptide reactivity exceeding by far that of ascaridole itself. Furthermore, activated ascaridole showed increased potential for induction of the Nrf2 target gene heme oxygenase 1 and upregulation of CD86 and CD54 on THP-1 cells, an established DC surrogate. These results indicate that radical formation could be involved in the steps leading to skin sensitization induced by the endoperoxide ascaridole.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26185204</PMID><DateCreated><Year>2015</Year><Month>09</Month><Day>26</Day></DateCreated><DateCompleted><Year>2016</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2015</Year><Month>09</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0929</ISSN><JournalIssue CitedMedium="Internet"><Volume>147</Volume><Issue>2</Issue><PubDate><Year>2015</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Toxicological sciences : an official journal of the Society of Toxicology</Title><ISOAbbreviation>Toxicol. Sci.</ISOAbbreviation></Journal><ArticleTitle>Activation of the Endoperoxide Ascaridole Modulates Its Sensitizing Capacity.</ArticleTitle><Pagination><MedlinePgn>515-23</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/toxsci/kfv148</ELocationID><Abstract><AbstractText>The monoterpene ascaridole, a fairly stable endoperoxide found in essential oils such as tea tree oil can provoke allergic contact dermatitis which has been evidenced under patch test conditions. However, concomitantly we observed irritative skin reactions that demand further data underlining the sensitization potential of ascaridole. Here, we studied the effects of ascaridole on dendritic cell (DC) activation and protein reactivity, 2 key steps of chemical-induced skin sensitization. Treatment of human monocyte-derived DC with ascaridole found support for full DC maturation, a capability of sensitizers but not irritants. It induced significant upregulation of the expression of the costimulatory molecules CD86, CD80, CD40, and the adhesion molecule CD54 in a time-dependent manner. Maturation was accompanied by release of proinflammatory cytokines interleukin (IL)-1ß, tumor necrosis factor-α, IL-6, and IL-8. Similar to other chemical skin sensitizers including hydroperoxides, we observed a certain reactivity of ascaridole toward cysteine- but not lysine-containing peptides. During recent years, evidence accumulated for a radical mechanism as trigger for protein reactivity of peroxides. Treatment of the fairly stable endoperoxide ascaridole with iron as radical inducer ("activated ascaridole") resulted in cysteine peptide reactivity exceeding by far that of ascaridole itself. Furthermore, activated ascaridole showed increased potential for induction of the Nrf2 target gene heme oxygenase 1 and upregulation of CD86 and CD54 on THP-1 cells, an established DC surrogate. These results indicate that radical formation could be involved in the steps leading to skin sensitization induced by the endoperoxide ascaridole.</AbstractText><CopyrightInformation>© The Author 2015. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Krutz</LastName><ForeName>Nora L</ForeName><Initials>NL</Initials><AffiliationInfo><Affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hennen</LastName><ForeName>Jennifer</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Korb</LastName><ForeName>Corinna</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schellenberger</LastName><ForeName>Mario T</ForeName><Initials>MT</Initials><AffiliationInfo><Affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerberick</LastName><ForeName>G Frank</ForeName><Initials>GF</Initials><AffiliationInfo><Affiliation>Central Product Safety Department, The Procter & Gamble Company, Mason Business Center, 8700 Mason Montgomery Road, Cincinnati, OH.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Blömeke</LastName><ForeName>Brunhilde</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>*Department of Environmental Toxicology, University Trier, 54296 Trier, Germany and bloemeke@uni-trier.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>07</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Toxicol Sci</MedlineTA><NlmUniqueID>9805461</NlmUniqueID><ISSNLinking>1096-0929</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019013">Antigens, CD40</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018122">Antigens, CD80</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051940">Antigens, CD86</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D039821">Monoterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010545">Peroxides</NameOfSubstance></Chemical><Chemical><RegistryNumber>126547-89-5</RegistryNumber><NameOfSubstance UI="D018799">Intercellular Adhesion Molecule-1</NameOfSubstance></Chemical><Chemical><RegistryNumber>1718D0GEVJ</RegistryNumber><NameOfSubstance UI="C043372">ascaridole</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019013" MajorTopicYN="N">Antigens, CD40</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018122" MajorTopicYN="N">Antigens, CD80</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051940" MajorTopicYN="N">Antigens, CD86</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003713" MajorTopicYN="N">Dendritic Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017449" MajorTopicYN="N">Dermatitis, Allergic Contact</DescriptorName><QualifierName UI="Q000209" MajorTopicYN="Y">etiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018799" MajorTopicYN="N">Intercellular Adhesion Molecule-1</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D039821" MajorTopicYN="N">Monoterpenes</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="Y">adverse effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010545" MajorTopicYN="N">Peroxides</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="Y">adverse effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012867" MajorTopicYN="N">Skin</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">THP-1</Keyword><Keyword MajorTopicYN="N">ascaridole</Keyword><Keyword MajorTopicYN="N">endoperoxide</Keyword><Keyword MajorTopicYN="N">monocyte-derived dendritic cells (MoDC)</Keyword><Keyword MajorTopicYN="N">skin sensitization</Keyword><Keyword MajorTopicYN="N">terpene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26185204</ArticleId><ArticleId IdType="pii">kfv148</ArticleId><ArticleId IdType="doi">10.1093/toxsci/kfv148</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><noCountry><name sortKey="Hennen, Jennifer" sort="Hennen, Jennifer" uniqKey="Hennen J" first="Jennifer" last="Hennen">Jennifer Hennen</name><name sortKey="Korb, Corinna" sort="Korb, Corinna" uniqKey="Korb C" first="Corinna" last="Korb">Corinna Korb</name><name sortKey="Krutz, Nora L" sort="Krutz, Nora L" uniqKey="Krutz N" first="Nora L" last="Krutz">Nora L. Krutz</name><name sortKey="Schellenberger, Mario T" sort="Schellenberger, Mario T" uniqKey="Schellenberger M" first="Mario T" last="Schellenberger">Mario T. Schellenberger</name></noCountry><country name="États-Unis"><region name="Ohio"><name sortKey="Gerberick, G Frank" sort="Gerberick, G Frank" uniqKey="Gerberick G" first="G Frank" last="Gerberick">G Frank Gerberick</name></region></country><country name="Allemagne"><noRegion><name sortKey="Blomeke, Brunhilde" sort="Blomeke, Brunhilde" uniqKey="Blomeke B" first="Brunhilde" last="Blömeke">Brunhilde Blömeke</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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